FastQCFastQC Report
Mon 27 Feb 2023
SRR3127946.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127946.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859637
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT598683.2193379675710907No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT44860.24122987443248334No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT44050.23687418566096502No Hit
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT37650.20245886697242527No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG27570.14825474003797515No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTC21800.11722717928283853No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC20420.10980637619062215No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19960.10733277515988335No Hit
CGTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC18730.1007185810994296No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT177350.090.2819061
TAGGGCA7650.072.4859854
ATAGGGC10550.072.159923
ATAGGGA16450.070.846653
GAATAGG10350.069.987871
AGTAGGG28150.069.947012
AGGGATG26350.069.735355
TATAGGG13850.068.8778842
GTACGGG3900.068.682132
GAGGGAT21050.066.7502064
GTAGGGA13100.066.0054863
GGTAAGG10350.065.897671
TAGGGAT11700.065.8705754
TAAGGGA16350.065.818993
AGGGATA15800.065.73435
GATAGGG24450.065.348192
CGTAGGG4100.065.331782
TAGTAGG6800.065.0221561
AGTAAGG10650.064.924731
ATAAGGG25200.064.895152