FastQCFastQC Report
Mon 27 Feb 2023
SRR3127945.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127945.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2301264
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT815993.545833941694651No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT44280.19241599399286652No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT43300.1881574647671888No Hit
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT36750.15969484596291428No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG28080.12201989862962268No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25200.10950503723171265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT241950.089.834811
ATAGGGC13050.076.712043
GAATAGG11650.073.885541
AGTAGGG37900.072.2975852
GTAGGGT7850.071.247853
TAGGGCA10550.070.8335654
ATAGGGA22050.070.7658843
AGGGATG34150.070.60425
TATAGGG19050.069.574262
TAGAGGG38700.066.552452
GAGGGAT25400.066.058534
ATAGCGG5200.066.03191
AATAGGG36350.065.8124162
GTACGGG5550.065.206692
TAGACGG2600.065.127351
AGAGGGC34500.064.98213
GGTAAGG11950.064.945691
GATAGGG31900.064.53242
TAGGGTA7650.064.509294
AGGGACT26200.064.401665
Produced by FastQC (version 0.11.5)