FastQCFastQC Report
Mon 27 Feb 2023
SRR3127944.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127944.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873199
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85700.9814486732119482No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39060.4473207138349906No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG37540.4299134561537519TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC33160.37975306888807703No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG31450.3601699039966834TruSeq Adapter, Index 22 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT13300.1523135047108391No Hit
AGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT12890.1476181259941892No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT11410.13066895404140408No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC10860.12437027527516636No Hit
GGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT9650.1105131819894434No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCGG9210.10547423897645325No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG450.094.082531
CGTTTTT29100.086.16151
TCCGCTA6950.085.960311
CGCTACG6900.084.460473
CGTAGGG1900.084.1212542
AAGGGTA5000.082.7167744
CTACGAC7250.081.031335
CGGGTAA700.080.568285
AGGGATG19800.080.466565
ATAGCGG1950.079.60831
ACGACCA7450.079.486837
AGAGGGC10250.079.323743
AGGGAAT20850.078.6683965
AACGGTC301.4561847E-678.330283
TAGGGTA3850.078.126824
GGATGGC15850.077.984347
TAGGGCG1150.077.649144
AGTAAGG4800.077.422091
GCTACGA7600.077.2996144
AGGACGG1400.077.282081
Produced by FastQC (version 0.11.5)