FastQCFastQC Report
Mon 27 Feb 2023
SRR3127943.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127943.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828224
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77800.9393594003554594No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG40960.4945521984390696TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC37480.45253458001700025No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35530.4289902248667027No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG34490.41643323545321076TruSeq Adapter, Index 22 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT14820.17893709914226102No Hit
AGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT13380.16155049841588748No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT12320.14875202843675142No Hit
GGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT11240.13571207789197123No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC10230.12351730932694535No Hit
ACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG10130.12230990649872497No Hit
AGAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT9750.11772177575148753No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCGG8840.10673441001468201No Hit
ACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT8640.10431960435824125No Hit
TAGACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT8500.10262924039873271No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG1150.085.827142
CGTAGGG1600.085.188542
CGTTTTT28400.084.60881
CGGGTAG452.7284841E-1183.551535
TAGGGCA4400.083.314174
CTACGAC6550.082.515115
TCCGCTA6450.082.381881
GGAATGC7750.081.867027
AAGGGTA5750.081.735194
AGGGATG20400.081.55495
CGCTACG6600.081.182813
GGATGGC16350.080.485427
AGGGTAT5850.080.3380055
GCGTAGG950.079.197111
AGGGAAT18650.078.875565
GATGACC8700.077.789358
TAAGGGA8450.077.314553
CGAAGGG5050.077.2484742
AACGGGC1650.076.910033
AAGGGCG3300.076.905394
Produced by FastQC (version 0.11.5)