FastQCFastQC Report
Mon 27 Feb 2023
SRR3127942.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127942.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990207
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111941.130470699560799No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45580.46030779422888346No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG40720.41122714745502703TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC38090.38466704436547106No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG35080.35426935984092217TruSeq Adapter, Index 22 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT15440.1559269930428688No Hit
AGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT13030.13158864762620343No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC12550.1267411763398966No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT11920.12037887027661894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG9500.086.5760653
AGGGATG26250.085.0447855
TCCGCTA9750.084.915381
CGTTTTT37950.084.661741
CTACGAC9900.083.078045
CGTAGGG1600.082.247262
TTACGGG1200.082.247262
AGGGTAT6400.081.512915
CGAAGGG6800.080.864952
GCTACGA10200.080.6345754
CGACCAA9600.080.2889948
ACGACCA9700.079.945797
TAGGGTA4000.079.897344
AGGGCGA3200.079.309865
GGATGGC22300.079.2447
TAGGGCA5750.078.466964
ATAGCGG1700.077.479611
GCATAGG2550.077.479611
AGGGCTA3950.077.3391955
AGGGAAT21000.076.987915
Produced by FastQC (version 0.11.5)