Basic Statistics
Measure | Value |
---|---|
Filename | SRR3127941.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2436265 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 53641 | 2.2017719747236035 | No Hit |
CGTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTT | 4736 | 0.19439592983521908 | No Hit |
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT | 3893 | 0.15979378269605318 | TruSeq Adapter, Index 10 (95% over 21bp) |
CGTTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT | 3597 | 0.147644037081352 | No Hit |
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG | 3310 | 0.13586370940763834 | RNA PCR Primer, Index 10 (95% over 22bp) |
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC | 2855 | 0.11718758016882401 | TruSeq Adapter, Index 10 (95% over 23bp) |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 2623 | 0.10766480657892305 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 16690 | 0.0 | 88.75066 | 1 |
AGGGATG | 4200 | 0.0 | 75.86761 | 5 |
ATAGGGA | 2105 | 0.0 | 74.124535 | 3 |
AGTAGGG | 3935 | 0.0 | 72.877716 | 2 |
ATAGGGC | 1420 | 0.0 | 72.15129 | 3 |
TAGGGCA | 1065 | 0.0 | 71.93064 | 4 |
TAGGGTA | 845 | 0.0 | 71.74786 | 4 |
AAGGGAT | 4360 | 0.0 | 69.41851 | 4 |
ACGGGTA | 170 | 0.0 | 69.11416 | 4 |
AAGGGTA | 1555 | 0.0 | 68.909706 | 4 |
GTAGGGT | 1005 | 0.0 | 68.742805 | 3 |
GTAGGGA | 1815 | 0.0 | 68.36018 | 3 |
ATAGCGG | 475 | 0.0 | 68.3516 | 1 |
GGTAAGG | 1420 | 0.0 | 67.92955 | 1 |
AGGGCAT | 1790 | 0.0 | 67.7396 | 5 |
TAGGGAT | 1995 | 0.0 | 67.375046 | 4 |
ATAAGGG | 3365 | 0.0 | 67.33977 | 2 |
GAATAGG | 1470 | 0.0 | 67.21948 | 1 |
TATAGGG | 2090 | 0.0 | 66.80667 | 2 |
AGAGGGC | 3465 | 0.0 | 66.73257 | 3 |