FastQCFastQC Report
Mon 27 Feb 2023
SRR3127941.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127941.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2436265
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT536412.2017719747236035No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTT47360.19439592983521908No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT38930.15979378269605318TruSeq Adapter, Index 10 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT35970.147644037081352No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG33100.13586370940763834RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC28550.11718758016882401TruSeq Adapter, Index 10 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26230.10766480657892305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT166900.088.750661
AGGGATG42000.075.867615
ATAGGGA21050.074.1245353
AGTAGGG39350.072.8777162
ATAGGGC14200.072.151293
TAGGGCA10650.071.930644
TAGGGTA8450.071.747864
AAGGGAT43600.069.418514
ACGGGTA1700.069.114164
AAGGGTA15550.068.9097064
GTAGGGT10050.068.7428053
GTAGGGA18150.068.360183
ATAGCGG4750.068.35161
GGTAAGG14200.067.929551
AGGGCAT17900.067.73965
TAGGGAT19950.067.3750464
ATAAGGG33650.067.339772
GAATAGG14700.067.219481
TATAGGG20900.066.806672
AGAGGGC34650.066.732573