SRR3127940.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3127940.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2270194
Sequences flagged as poor quality	0
Sequence length	100
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48868	2.1525913644384578	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTT	5128	0.22588377909553103	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT	4080	0.17972032346134295	TruSeq Adapter, Index 10 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT	3929	0.17306890952931775	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	3662	0.1613078001263328	RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	3160	0.13919515248476563	TruSeq Adapter, Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	2479	0.10919771614232088	RNA PCR Primer, Index 35 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC	2353	0.10364752968248529	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15070	88.42657	1
AGGGATG	3865	75.147606	5
TAGGGCA	1000	74.72298	4
ACGGGTA	310	74.28334	4
GAATAGG	1390	72.41831	1
GAGGGAT	2700	71.36366	4
AGTAGGG	3370	70.284195	2
ATAGCGG	470	70.05669	1
ATAGGGA	1960	69.29451	3
ATAGGGC	1415	69.08185	3
AGGGCAT	1790	69.05242	5
AGGGAAT	2595	68.2779	5
ACGGGAT	725	68.06257	4
AGGGTAG	915	67.7999	5
TAGGGTA	705	67.32701	4
TAAGGGA	1965	67.20488	3
TAGGGTC	620	66.70341	4
AGAGGGC	3120	66.42645	3
TAGGGCG	455	66.103676	4
ATAAGGG	3290	65.708115	2