FastQCFastQC Report
Mon 27 Feb 2023
SRR3127939.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127939.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2824693
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT693932.4566563516814037No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTT47260.16731021742893828No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT37920.13424467720916927TruSeq Adapter, Index 10 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT37420.13247457334301463No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG35290.12493393087319576RNA PCR Primer, Index 10 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30680.10861357322724983No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC30030.10631243820124878TruSeq Adapter, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT207950.088.327151
AGGGATG49000.076.4454655
TAGGGCA13100.071.754354
ATAGCGG7350.071.692551
AGAGGGC41000.070.498653
AGTAGGG44750.069.632182
TAAGGGA26000.069.594823
GAGGGAT38500.069.094784
TAGGGTA10000.068.618684
AGGGTAG12150.068.467825
ATAGGGC17050.068.086663
GAGGGTA14450.067.652684
ATAGGGA26400.067.650223
GAATAGG16700.067.3325041
ACGGGTA2800.067.141574
TATAGGG24950.067.060842
TAGAGGG50400.066.7685552
AAGGGAT51950.066.495354
AGATAGG15800.066.105751
AGGGAAT32800.066.056665