Basic Statistics
Measure | Value |
---|---|
Filename | SRR3127939.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2824693 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 69393 | 2.4566563516814037 | No Hit |
CGTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTT | 4726 | 0.16731021742893828 | No Hit |
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT | 3792 | 0.13424467720916927 | TruSeq Adapter, Index 10 (95% over 21bp) |
CGTTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT | 3742 | 0.13247457334301463 | No Hit |
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG | 3529 | 0.12493393087319576 | RNA PCR Primer, Index 10 (95% over 22bp) |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 3068 | 0.10861357322724983 | No Hit |
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC | 3003 | 0.10631243820124878 | TruSeq Adapter, Index 10 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 20795 | 0.0 | 88.32715 | 1 |
AGGGATG | 4900 | 0.0 | 76.445465 | 5 |
TAGGGCA | 1310 | 0.0 | 71.75435 | 4 |
ATAGCGG | 735 | 0.0 | 71.69255 | 1 |
AGAGGGC | 4100 | 0.0 | 70.49865 | 3 |
AGTAGGG | 4475 | 0.0 | 69.63218 | 2 |
TAAGGGA | 2600 | 0.0 | 69.59482 | 3 |
GAGGGAT | 3850 | 0.0 | 69.09478 | 4 |
TAGGGTA | 1000 | 0.0 | 68.61868 | 4 |
AGGGTAG | 1215 | 0.0 | 68.46782 | 5 |
ATAGGGC | 1705 | 0.0 | 68.08666 | 3 |
GAGGGTA | 1445 | 0.0 | 67.65268 | 4 |
ATAGGGA | 2640 | 0.0 | 67.65022 | 3 |
GAATAGG | 1670 | 0.0 | 67.332504 | 1 |
ACGGGTA | 280 | 0.0 | 67.14157 | 4 |
TATAGGG | 2495 | 0.0 | 67.06084 | 2 |
TAGAGGG | 5040 | 0.0 | 66.768555 | 2 |
AAGGGAT | 5195 | 0.0 | 66.49535 | 4 |
AGATAGG | 1580 | 0.0 | 66.10575 | 1 |
AGGGAAT | 3280 | 0.0 | 66.05666 | 5 |