FastQCFastQC Report
Mon 27 Feb 2023
SRR3127938.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127938.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761388
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760934.320058953507121No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT68080.38651336332483244No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT66010.3747612678183342No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT54460.3091879812965684RNA PCR Primer, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG31780.18042589139928283RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTC30940.17565692510679076No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC24750.14051418540378385RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC20750.1178048221062026RNA PCR Primer, Index 16 (96% over 25bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18680.10605272659970433No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18240.1035546966369704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT194050.090.533341
ATAGGGC11050.076.555283
TAGGGCA7800.072.904734
GAGGGTA10050.070.6115654
AGTAGGG25650.070.590562
AGGGATG21200.069.829615
AGTAAGG10450.069.822591
GTAGGGT6400.069.760343
AAGGGTA12150.069.624354
ACGGGTA2300.069.472994
TAGGGTC5150.069.3539664
AGGGTAC7000.069.15195
GAATAGG8800.069.006131
TACGGGT1500.068.928113
ATAGCGG4450.068.75971
TAGAGGG31400.068.7474062
AGATAGG10750.068.311951
GTTTTTT263250.067.333472
TATAGGG14050.066.946352
CGTTTCT10350.066.858651
Produced by FastQC (version 0.11.5)