Basic Statistics
Measure | Value |
---|---|
Filename | SRR3127936.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2051570 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 94840 | 4.622801074299195 | No Hit |
CGTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT | 7333 | 0.35743357526187264 | No Hit |
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT | 7278 | 0.3547527015895144 | No Hit |
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT | 5579 | 0.2719380766924843 | RNA PCR Primer, Index 16 (95% over 23bp) |
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG | 3593 | 0.1751341655415121 | RNA PCR Primer, Index 16 (95% over 24bp) |
CGTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTC | 3557 | 0.17337941186505945 | No Hit |
CGTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC | 2665 | 0.12990051521517668 | RNA PCR Primer, Index 16 (95% over 22bp) |
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2394 | 0.11669111948410242 | No Hit |
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC | 2144 | 0.10450533006429222 | RNA PCR Primer, Index 16 (96% over 25bp) |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 2122 | 0.10343298059534893 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 24765 | 0.0 | 90.60114 | 1 |
ACGGGTA | 225 | 0.0 | 75.20035 | 4 |
ATAGGGC | 1295 | 0.0 | 73.67602 | 3 |
TAGGGCA | 890 | 0.0 | 72.87675 | 4 |
GAATAGG | 1295 | 0.0 | 72.29828 | 1 |
TAGACGG | 295 | 0.0 | 71.768715 | 1 |
AGGGATG | 2510 | 0.0 | 71.71747 | 5 |
AGTAGGG | 2875 | 0.0 | 69.80724 | 2 |
AGGGTAC | 890 | 0.0 | 69.70819 | 5 |
GTACGGG | 425 | 0.0 | 69.672615 | 2 |
ATAGAGG | 1500 | 0.0 | 69.004295 | 1 |
ATAGCGG | 475 | 0.0 | 68.34397 | 1 |
TATAGGG | 1700 | 0.0 | 68.29022 | 2 |
TAGAGGG | 3790 | 0.0 | 68.20779 | 2 |
GTTTTTT | 33295 | 0.0 | 67.46344 | 2 |
ATAGGGA | 1765 | 0.0 | 67.371414 | 3 |
GAGGGAT | 2055 | 0.0 | 67.24119 | 4 |
TAGGGTC | 695 | 0.0 | 66.27369 | 4 |
AGAGGGC | 2920 | 0.0 | 66.15442 | 3 |
AGTAAGG | 1195 | 0.0 | 66.143326 | 1 |