FastQCFastQC Report
Mon 27 Feb 2023
SRR3127936.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127936.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2051570
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT948404.622801074299195No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT73330.35743357526187264No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT72780.3547527015895144No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT55790.2719380766924843RNA PCR Primer, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG35930.1751341655415121RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTC35570.17337941186505945No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC26650.12990051521517668RNA PCR Primer, Index 16 (95% over 22bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23940.11669111948410242No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC21440.10450533006429222RNA PCR Primer, Index 16 (96% over 25bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21220.10343298059534893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT247650.090.601141
ACGGGTA2250.075.200354
ATAGGGC12950.073.676023
TAGGGCA8900.072.876754
GAATAGG12950.072.298281
TAGACGG2950.071.7687151
AGGGATG25100.071.717475
AGTAGGG28750.069.807242
AGGGTAC8900.069.708195
GTACGGG4250.069.6726152
ATAGAGG15000.069.0042951
ATAGCGG4750.068.343971
TATAGGG17000.068.290222
TAGAGGG37900.068.207792
GTTTTTT332950.067.463442
ATAGGGA17650.067.3714143
GAGGGAT20550.067.241194
TAGGGTC6950.066.273694
AGAGGGC29200.066.154423
AGTAAGG11950.066.1433261