FastQCFastQC Report
Mon 27 Feb 2023
SRR3127935.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127935.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351737
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126780.9379043408592056No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC83400.6169839251274472No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG33030.24435226675011487No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC31740.23480899021037374No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG26740.19781954625788892No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC22810.16874584331123585No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGCT18340.1356772804177144No Hit
AAAGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT18040.1334579137805653No Hit
TAGACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT13930.10305259085162276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATG52850.084.660155
GGATGGC48000.084.4999857
CGTTTTT41850.083.864951
CGGGTAT1550.081.868945
GATGGCA47100.081.62438
AAGGGAT39150.080.792384
CGCTACG16300.080.1575243
ATAGCGG3000.079.980861
CGGGAAT5700.079.980485
GGAATGC15750.079.972617
GCTACGA16350.079.9123844
ATGGCAC48150.079.649129
GCGGGAT8150.079.004174
TAAGGGA16950.078.747313
TACGAGG2100.078.412611
GGGATGG56100.078.163836
GTAGGGT4100.077.949293
CGGGATG9900.077.856665
AGGGTAT10600.077.5924155
CGAGGGA9650.077.438493