FastQCFastQC Report
Mon 27 Feb 2023
SRR3127933.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127933.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607953
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167521.0418214960263141No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC107390.667867779717442No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG33720.20970762205114205No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC30300.18843834365805467No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG27510.171087090232115No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC25920.1611987415055042No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGCT20470.12730471599605211No Hit
AAAGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT18290.1137471057922713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51400.085.243911
GGATGGC57350.084.3265157
AGGGATG63800.083.536185
AGGGAAT33300.081.012095
TACCGAT353.8666258E-880.568539
GATGGCA57450.080.171178
GCGGGAT11200.080.153884
CGTAGGG4300.079.792782
CGCTACG19100.079.7298363
AAGGGAT50000.079.526084
AAGGGCG7750.079.447244
CGGGATG13850.079.4050755
GCTACGA18950.079.3688354
GGGATGG68200.078.077836
ATTGCGG1150.077.755761
TAGCGGG6350.077.7185752
ATAGCGG2850.077.612161
AGGGCAT18600.077.572475
ATGGCAC59300.077.5115369
GGGCGAT6250.077.453216
Produced by FastQC (version 0.11.5)