FastQCFastQC Report
Mon 27 Feb 2023
SRR3127932.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127932.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952650
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82390.8648506796829896No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC52550.5516191675851572No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG26600.2792211200335905No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC24370.2558127329029549No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG22960.24101191413425704No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTGG12470.1308980213089802No Hit
AAAGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTT11220.117776728074319No Hit
TAGACCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCT10580.11105862593817246No Hit
AGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCT10510.11032383351703143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA600.093.9962844
CGCTACG10100.086.5510253
CTACGAC10150.085.661635
TACGAGG2600.085.056381
TCCGCTA10450.084.6494141
CGTTTTT30800.084.175031
GCTACGA10500.082.8062444
ATAGCGG1950.082.04021
AGGGATG29950.081.442515
TAACGGG2750.080.370482
TAGGGCG2400.080.288494
TACGACC10850.080.135076
ACGACCA10600.079.364787
AAGGGAT25050.078.986894
TAAGGGA12500.077.452933
AGCGGGA6700.077.1611253
GGATGGC20250.077.0537347
TACGCGG551.8189894E-1276.994941
AGGGATC10750.076.9457865
TACGGGT551.8189894E-1276.906043
Produced by FastQC (version 0.11.5)