FastQCFastQC Report
Mon 27 Feb 2023
SRR3127930.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127930.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102807
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106470.9654454496571023No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC62560.5672796781304436No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG28020.254078909546276No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG25290.22932389801660671No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC23390.2120951354135402No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTGG11780.10681832813901253No Hit
AAAGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTT11160.1011963108685382No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT37900.086.414341
TAGGGCG2300.085.82724
CGCTACG12050.084.250053
GTACGGG2350.084.001092
AGGGATG29400.082.9667055
TAGACGG800.082.3481451
ATAGCGG2850.080.903441
GCTACGA12500.080.841054
CGTAGGG2950.079.6620562
GGATGGC22550.078.573977
AGGGCAT9400.078.497475
ACGGGTC900.078.334364
TCCGCTA13300.078.190941
AGTACGG2050.078.044241
TACGACC12900.077.966486
AAGGGAC12450.077.768084
CTACGAC13050.077.430455
TAGGGCA5950.076.622854
ACGACCA12750.076.303417
TACGAGG2100.076.186041
Produced by FastQC (version 0.11.5)