FastQCFastQC Report
Mon 27 Feb 2023
SRR3127929.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127929.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2560937
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226130.882997121756607No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32860.12831241065281965No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG29750.11616841804386441Illumina PCR Primer Index 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG25980.10144724372368394RNA PCR Primer, Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76100.081.508261
ATAGGGC14900.073.493113
TAGGGCA12350.071.543114
ATGCGGG14500.069.393332
AGGGCAT18850.069.062995
AGTAGGG43250.067.294032
TAGAGGG48100.067.1558152
GAATAGG16150.067.023361
AATGCGG6450.066.397631
ATAGGGA25450.066.295433
AGAGGGC36500.065.925583
ACGGGAT7350.065.860774
AGTAAGG15500.065.583251
CGAGGGA13600.065.3130343
AAGAGGG91750.065.032072
AAGGGAC24250.064.9247064
AAGGGTC18200.064.815564
GATAGGG37100.064.761812
AAGGGGC70700.064.214734
CGTAGGG6900.064.0546652
Produced by FastQC (version 0.11.5)