FastQCFastQC Report
Mon 27 Feb 2023
SRR3127928.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127928.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375317
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203040.8547911710310666No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG30960.13034049771041084Illumina PCR Primer Index 2 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28150.11851049775672047No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG27620.11627921662666499RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC25760.10844868284948914TruSeq Adapter, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65350.081.4022751
AGTAGGG39000.067.722752
GAATAGG15350.067.4114761
TAACGGG6800.067.038712
ATAGGGC16450.066.851753
TAGGGTA7750.066.70434
ATAGGGA21450.066.605353
AGTAAGG17250.066.530421
ATGCGGG12300.066.4824452
TAGGGCA10800.066.1428154
CGTAGGG6650.065.724022
AGGGATG33500.065.655795
TAGAGGG46700.065.613552
AAGAGGG86600.065.121772
GAGGGAT31850.064.7765664
AGGGACT31300.064.4146965
ATGAGGG38200.064.342982
TAGGGTC7250.064.174144
GATAGGG33650.064.104792
ATAGAGG17100.064.088391
Produced by FastQC (version 0.11.5)