FastQCFastQC Report
Mon 27 Feb 2023
SRR3127927.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127927.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2964066
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290670.9806461799433616No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37040.1249634792207731No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG30510.10293293064324478Illumina PCR Primer Index 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94550.082.555911
ATAGGGC18200.073.084263
ATGCGGG14250.068.605252
TAGAGGG55800.068.3959962
ATAGGGA28200.068.335073
AGTAGGG52800.068.009292
AGATAGG14650.067.12311
TAGGGCA12500.066.929694
TAAGGGA25350.066.5618443
ATAGAGG20150.065.847211
AAGAGGG110100.065.656522
TAGTAGG14200.065.605491
AGGGAAT30200.065.206965
AATAGGG48700.065.1453862
TAGCGGG17050.065.0573652
TAACGGG9700.064.9294742
TATAGGG24600.064.578872
AGAGGGC43400.064.545423
AATGCGG7250.064.248131
ACGGGAT9700.063.9603774
Produced by FastQC (version 0.11.5)