FastQCFastQC Report
Mon 27 Feb 2023
SRR3127926.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127926.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253012
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182260.8089615146301928No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36290.161073265477503No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG31090.13799305108006527No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG30380.13484171411426127No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC26570.11793101856536939No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT2750.083.74135
CGTTTTT62450.083.715841
CGTAGGG6050.076.17412
TAGGGTA10600.074.9302444
ATAGGGC15200.073.5884553
AGGGATG41000.072.90375
AGTAGGG37800.072.404882
ATAGCGG4750.072.319481
TAGCGGG11000.072.24882
TAGGGCA11950.071.971334
GTAGGGT9950.071.795433
AGGGCGA14900.071.600485
AGGGAAT28950.071.105285
ATAGGGA19650.070.317123
AAGGGCG11050.070.177514
AAGAGGG74300.069.747632
ACGGGTA2400.068.538274
AGAGGGC29300.068.4914863
TAAGGGA19450.068.382213
GAGGGAT36200.068.289444
Produced by FastQC (version 0.11.5)