FastQCFastQC Report
Mon 27 Feb 2023
SRR3127925.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127925.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145359
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167070.7787507825030683No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35040.16332930758907951No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG32750.15265510341159685No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG32270.15041771563640396No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC28420.1324720011895445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53100.084.600911
CGTAGGG5300.076.2577062
TAGGGCA10300.075.283144
ATAGGGC13850.074.650733
AGTAGGG37800.072.2346342
TAGGGTA8750.071.9693764
AGGGATG36850.071.928795
ATAGCGG4650.071.824331
GTAGGGT9700.071.220793
TAGCGGG11300.071.1178742
CTACGAC8500.070.21765
AAGGGAT37750.070.21234
AGGGAAT27200.069.975715
GAGGGAT32050.069.9425354
AAGGGCG11100.069.857334
AGGGTAC12600.069.748065
AGGGCGA14700.069.375095
TAAGGGA21000.068.479933
GAATAGG15250.068.477731
GGGCGAT10150.068.06486
Produced by FastQC (version 0.11.5)