FastQCFastQC Report
Mon 27 Feb 2023
SRR3127924.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127924.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2643898
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238830.9033253173912156No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46530.1759901478801376No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG32880.1243618324156227No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG31320.11846145350539242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77800.084.5899661
CGTAGGG8150.080.161932
ATAGGGC16050.074.9679953
ACGGGTA3450.074.9297944
AGGGATG50150.074.598225
ATAGCGG5900.074.1500851
TAAGGGA25100.072.655583
TAGCGGG13650.072.6542052
AAGGGAT50350.072.439134
TAGGGCA12650.071.338124
TAGGGTA12400.071.2602164
AGTAAGG19000.070.809761
GAGGGTA19600.070.262334
AGGGCGA15350.070.11545
GAGGGAT40250.069.830684
AGTAGGG44700.069.398072
CGGGTAT3550.068.843245
AAGGGCG11500.068.662944
ATAGGGA24950.068.006063
AAGAGGG88800.067.802722
Produced by FastQC (version 0.11.5)