FastQCFastQC Report
Mon 27 Feb 2023
SRR3127923.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127923.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516351
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58231.1277212593758896No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22690.43942976773551323No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG22410.43400709982163294No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG19920.38578408873034037No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC17600.340853411729618TruSeq Adapter, Index 14 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT7170.13885903193757734No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT6250.1210416945062564No Hit
AAAGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT6210.12026702766141635No Hit
ACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG5910.11445702632511606TruSeq Adapter, Index 14 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT5640.10922802512244578No Hit
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT5220.10109402325162535No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGG409.094947E-1294.115381
CTATCGG156.8664795E-494.115371
TATCGGG254.9069604E-794.0333252
TAACGGG850.088.501952
TGTGACG800.088.122095
CGTTTTT19200.086.762611
AATCGGG401.031367E-982.279152
AGGGCGT750.081.463985
GCGGGTA700.080.568774
AGGGCAT4450.080.267015
AGGGATG11150.080.087045
AGGGCGA2350.079.997365
TCCGCTA4500.079.475211
AGGGAAT10650.078.99275
AGTAAGG2800.078.989691
GAGGGAT8300.078.708254
GCTACGA4600.078.671324
GGGCGAA301.4552861E-678.330756
AGGGTAC2050.077.9486545
GGGCGAT2050.077.9486546
Produced by FastQC (version 0.11.5)