FastQCFastQC Report
Mon 27 Feb 2023
SRR3127922.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127922.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491066
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53381.0870229256352508No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG24150.49178725466637885No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG21650.44087760097420714No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21000.42764109101424247No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC19270.39241161065925967TruSeq Adapter, Index 14 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT7510.15293259969128387No Hit
AAAGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT7070.14397250064146164No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT6710.1366415105097889No Hit
GGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT6670.13582695605071415No Hit
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT6250.12727413423042933No Hit
ACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG6110.12442319362366769TruSeq Adapter, Index 14 (95% over 21bp)
AGAGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT5710.11627764903292023No Hit
GAATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT5320.10833574305694144No Hit
GAAGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT5130.10446660937633637No Hit
GATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC4990.10161566876957476No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCACC4980.10141203015480607No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG4930.10039383708096265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGG254.8989386E-794.057981
ACGACGG201.8312276E-594.057981
CCACGCA156.902484E-493.9909494
TTACGGG500.093.981362
GTCGAGG600.086.219821
CGTTTTT18000.086.219821
CGCTACG3350.085.5651253
GCTACGA3350.085.5651254
TACGAGG452.7284841E-1183.60711
GTTACGG401.029548E-982.3007351
AAGGGCG2350.079.9841464
AGACGGG2300.079.6798552
GGATGAC4750.079.158327
TAGGGCA2150.078.6820764
CGGGAAC600.078.325785
ATGGGCG600.078.31784
CGAGGGA2650.078.022273
ATAGCGG1150.077.700071
AGGGAAT12050.077.610785
TCCGCTA3700.077.534291
Produced by FastQC (version 0.11.5)