FastQCFastQC Report
Mon 27 Feb 2023
SRR3127921.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127921.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594504
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78351.3179053463054917No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27190.45735604806696006No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG23990.4035296650653318No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG20820.3502079044043438No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC18600.3128658511969642TruSeq Adapter, Index 14 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT8600.14465840431687593No Hit
AAAGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT6960.11707238302854145No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT6390.1074845585563764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT22700.085.392591
CGCTACG5200.084.9568253
TCCGCTA5150.084.04841
CGAGGGA3550.083.4038243
GTTACGG401.02591E-982.3354641
GCTACGA5400.081.810274
TAGCGGG2250.081.462152
CTACGAC5400.080.939955
AGGGATG12800.079.30815
AGGGCGT950.079.15355
TAAACGG1200.078.414721
TGTGACG900.078.328995
TTACGGG850.077.407472
GCGGGAT2750.076.904824
AAGGGTA5200.076.822664
AGTAGGG8850.076.470332
ACGGGCC800.076.3707664
TACGACC5750.076.0131766
GAGGGAT10700.075.986444
AGGGAAT13750.075.8794255
Produced by FastQC (version 0.11.5)