FastQCFastQC Report
Mon 27 Feb 2023
SRR3127920.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127920.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2912510
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358611.231274742404318No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41520.14255745044652207No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35390.12151031241094451No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG33720.11577642651870723No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG32370.1111412493004316No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC29670.1018708948638803No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT111800.085.513421
ATAGGGC16550.076.388723
AGGGATG51300.075.48925
GTACGGG5550.075.399052
ATAGCGG5000.074.337751
GAATAGG19950.074.288231
CGTAGGG9150.072.968612
TAGGGTA13350.071.816444
TAGGGCA14850.071.524854
AGTAAGG18600.071.332681
ATAGGGA30150.070.924953
TAAGGGA27850.070.538473
AGATAGG16750.069.660921
GAGGGAT37850.069.1614764
TAGGGAA16450.069.1392064
GTAGGGA22500.068.9296953
AGTAGGG50450.067.848312
AGGGAAT33600.067.419065
AAGGGAT51050.067.1130144
ATAAGGG45700.066.9782262
Produced by FastQC (version 0.11.5)