FastQCFastQC Report
Mon 27 Feb 2023
SRR3127918.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127918.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3362270
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460911.37082982627808No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46790.13916193524018No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38480.1144464900201352No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG38170.11352449386872558No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG33740.10034887144696886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT129400.085.547941
TAGGGTA13350.076.749784
AGGGATG61950.075.869475
AGTAAGG22000.072.279641
ATAGGGA34150.072.1178053
AGGGTAC18650.072.076815
GAATAGG22500.071.927981
AGTAGGG58950.071.9158552
ATAGGGC20800.071.856443
ATAGCGG6550.071.107571
TAAGGGA30850.070.9958043
TAGGGCA17500.070.366374
CGTAGGG10800.070.065472
TAGGGAT26300.069.517754
AGGGAAT36100.069.3950355
GTACGGG7000.069.157792
ATAAGGG52900.067.879652
GAGGGAT44750.067.848684
GTAGGGT14400.067.5631263
GGATGGC52950.067.550267
Produced by FastQC (version 0.11.5)