FastQCFastQC Report
Mon 27 Feb 2023
SRR3127917.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127917.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737361
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT651663.7508612199767346No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT37910.21820450672024985No Hit
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT36630.21083701084575976RNA PCR Primer, Index 34 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCT32320.1860292708308751No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG23770.13681670073174199RNA PCR Primer, Index 34 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21620.1244416100050594No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT187650.090.096621
AGGGATG28050.073.723665
ATAGGGC9700.073.647663
ATAGGGA16450.073.426753
TAGGGAT12400.073.151414
AGTAGGG28000.071.704182
AGGGTAC8000.071.673225
ACGGGAT4850.069.771474
GTAGGGT6150.069.542993
GAGGGAT20500.069.466564
TAGGGTC4200.069.379234
AGGGCAT12350.069.2614445
AGATAGG10550.068.685981
GTACGGG4150.067.979472
TAGGGCA7500.067.678324
AGAGGGC25700.067.480823
TAGGGTA6500.067.2444844
GAATAGG10750.066.970391
GTAGGGA13200.066.581693
GTTTTTT259800.065.986052
Produced by FastQC (version 0.11.5)