FastQCFastQC Report
Mon 27 Feb 2023
SRR3127916.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127916.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648750
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610253.7012888551933285No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT41000.2486732373009856No Hit
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT39730.2409704321455648RNA PCR Primer, Index 34 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCT35810.21719484457922666No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG25700.1558756633813495RNA PCR Primer, Index 34 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19850.1203942380591357No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC17500.10614101592115238RNA PCR Primer, Index 34 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTC17100.10371493555724033No Hit
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC16690.10122820318423048RNA PCR Primer, Index 34 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT176350.090.179571
ATAGGGC10000.078.477753
TAGGGTC4550.073.329214
TACGGGT1750.072.502963
AGGGATG28350.072.436665
TAGGGCA7500.070.1757054
GTAGGGC8600.068.849723
TAGGGAT10300.067.9796754
TAAGGGA13500.067.18213
GAGGGAT19600.066.892624
AGTAGGG27550.066.6937562
TAGGGTA4750.066.284384
AAGGGTA12050.065.9067154
TATAGGG13050.065.897762
TAGAGGG30400.065.8515552
GTTTTTT244150.065.8070452
AGATAGG8950.065.693961
AGGGCAT12450.065.676495
AGGGACT15550.065.578185
GAATAGG10150.065.341871