FastQCFastQC Report
Mon 27 Feb 2023
SRR3127914.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127914.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2371990
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252141.0629893043393945No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37480.15801078419386255No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG30140.12706630297766855No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29080.12259748143963509No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG26070.10990771461937023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72250.082.8403551
ATAGGGC15400.076.295523
TAGGGCA11100.074.096014
AGTAGGG39100.070.8274152
AGATAGG12600.070.206941
AGGGAAT27650.069.859655
GTACGGG4850.069.799712
GAATAGG14050.069.65941
AGGGATG31950.069.4305955
AGTTAGG7350.069.139971
TATAGGG19050.068.86082
AGGGCGA10250.068.31915
TAGGGTC8350.068.104944
GTAGGGA18950.067.954953
ATAGGGT12450.067.9489753
GTAGGGT11500.067.8406453
TAGGGCG4450.067.592684
ACTAGGG12700.067.379922
ATAGAGG14250.067.360921
AATAGGG35050.067.2066962
Produced by FastQC (version 0.11.5)