FastQCFastQC Report
Mon 27 Feb 2023
SRR3127913.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127913.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283784
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231121.0120046379167207No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36030.1577644821051378No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG34350.15040826978383245No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG29730.13017868590024276No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26420.1156851961481471No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC26050.11406507795833581No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60400.083.791711
TAGGGCG4300.078.6898654
TAGGGCA10500.077.877984
ATAGGGC14850.076.901463
GAATAGG13800.070.894081
ACGGGTA2600.070.4930044
ATAGGGA21650.070.113143
AGGGATG31400.069.595015
ATAGCGG4800.069.573341
AGATAGG12850.069.180621
AGTAGGG38250.069.172222
TATAGGG16850.068.8893052
CGTAGGG6400.068.29012
AAGGGAC22350.067.7069244
AGGGAAT25100.067.59095
ATGCGGG8150.067.46572
GTAGGGT10450.067.457423
AATAGGG32650.067.362392
ACTAGGG11450.066.9016652
CGAAGGG22700.066.455962
Produced by FastQC (version 0.11.5)