FastQCFastQC Report
Mon 27 Feb 2023
SRR3127912.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127912.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793650
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324701.1622787392837328No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45190.16175970504537077No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34090.12202673921214181No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG32530.11644264671666099No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30030.10749378053800585No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG28200.10094321049523025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94000.083.6240841
ATAGGGC18600.079.094943
TAGGGCA13650.075.065634
CGTAGGG7150.072.96832
AGTAGGG49350.072.669922
TAGGGCG6550.072.4765244
AGTACGG3550.071.556351
GTAGGGT12550.071.533083
TAGGGTA11750.071.2031864
GTACGGG6050.070.697372
AGGGATG35950.070.205215
CTACGAC11350.069.1535645
ACGGGAT9550.067.919274
ATAGAGG17000.067.795361
TAAGGGA25650.067.250573
GAATAGG17300.067.163551
AAGGGAC24650.067.118634
AGGGTAC14400.066.909135
GTAGGGA22500.066.8474353
GAGGGAT33300.066.762754
Produced by FastQC (version 0.11.5)