FastQCFastQC Report
Mon 27 Feb 2023
SRR3127911.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127911.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693704
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356821.3246444301229832No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC48570.1803093435655885No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35800.1329025015369172No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG29340.10892065349422209No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT101650.086.509391
AGGGATG49500.076.240855
AGGGAAT29700.073.424155
TAGGGCA14600.073.393794
GTAGGGT11600.072.117293
AAGGGTA19250.072.022574
ATAGGGA28000.071.839463
GAGGGAT37100.071.700124
AGGGTAC13050.071.306985
TAGGGTC8500.070.773134
ATAGGGC19900.069.906253
TAGGGTA10700.068.9593664
CGTAGGG7500.068.3224642
AGTAGGG46500.068.140492
GAATAGG20100.067.4151841
GTAGGGA22750.066.313353
AGGGTAA14400.065.9274445
AGGGCAT23900.065.875555
AAGAGGG92500.065.76442
GTAAAGG21600.065.347481
Produced by FastQC (version 0.11.5)