FastQCFastQC Report
Mon 27 Feb 2023
SRR3127910.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127910.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2515270
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326131.2966003649707587No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45580.18121315007931554No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31910.12686510792081965No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG30930.1229689059226246No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG29530.11740290306806028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95550.085.0605851
TAGGGTA9200.076.61944
AGGGATG46950.075.9714055
AGGGAAT27950.074.148225
ATAGGGC16150.073.035923
GAGGGAT31900.072.625914
ATAGGGA26750.071.499983
GAATAGG18500.071.44141
AGGGTAA14650.070.5714345
TAGGGCA12600.070.116884
AGTAGGG43650.069.8708042
GTAGGGA21950.069.7940443
GTAGGGT10650.069.2764053
AGGGTAC11250.068.9247745
ATAGAGG18500.068.390521
CTACGAC10950.068.2381065
AAGGGTA19200.067.797524
ATAAGGG33100.066.869522
TATAGGG20750.066.583182
AAGGGAT50350.066.3598564
Produced by FastQC (version 0.11.5)