FastQCFastQC Report
Mon 27 Feb 2023
SRR3127909.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127909.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3145268
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT467781.4872500530956345No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC57890.18405426818954698No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41070.1305771082146259No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT129750.085.741551
AGGGATG53900.077.34455
ATAGGGC21600.076.374423
TAGGGCA16300.074.680414
AGGGAAT35350.073.657145
AGGGTAC14300.071.978485
ATAGGGA32200.071.0833053
GAGGGAT38300.070.928964
ATAGCGG6000.070.560041
GAATAGG21900.070.237841
CGTAGGG9600.070.009892
AAGGGTA23300.069.390044
AGTAGGG52400.069.154052
TAGGGTA12450.067.9512944
ATAAGGG44300.067.4757842
TATAGGG26300.067.372112
TAGAGGG57400.067.142342
AGGGTAG16100.066.850425
ATAGAGG20300.066.273141
AAGGGAT62550.066.272874
Produced by FastQC (version 0.11.5)