FastQCFastQC Report
Mon 27 Feb 2023
SRR3127908.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127908.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043613
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436211.433197978849479No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG31230.10260831452618976No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG31140.1023126133315898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT138750.086.825671
GAATAGG18500.069.443571
ATAGGGA28250.069.372543
CGTAGGG7050.069.358512
ATAGGGC17850.068.9815753
AGGGATG39950.068.936755
AGTAGGG52050.068.741572
AGGGCAT24950.068.5647355
AAGGGAT50150.068.035524
TAGGGCG6300.067.884544
GAGGGAT40050.066.417724
AGAGGGC43050.065.828533
TAAGGGA28000.065.627933
AGGGAAT36650.065.526495
TAGGGCA16900.065.350844
AATAGGG46750.064.868472
TAGAGGG53700.064.615642
ATGCGGG13400.064.560752
AAGAGGG99900.064.5249252
AGATAGG17300.064.195861
Produced by FastQC (version 0.11.5)