FastQCFastQC Report
Mon 27 Feb 2023
SRR3127907.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127907.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804749
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398561.4210184226823863No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG35270.12575100303093076No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG33090.11797847151385026No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC31280.11152513112581554TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT126100.086.377711
CGTAGGG8400.076.0860062
TAGGGCA12150.071.941884
ATAGGGC16150.071.873633
ATAGCGG4900.068.146011
GAATAGG16150.067.851841
ACGGGAT7000.067.1347054
AGGGATG37700.066.6918955
ATAGAGG17500.066.3799741
AATAGGG40300.066.118522
TAAGGGA21500.066.01063
ATAGGGA24350.066.004313
AGTAGGG42400.065.725522
GTACGGG7250.065.467912
AGTAAGG17100.065.457441
TAACGGG8300.065.112582
AGATAGG14450.065.09391
AAGAGGG92750.064.550652
AGAGGGC39650.064.357883
TATAGGG21050.064.2962342
Produced by FastQC (version 0.11.5)