FastQCFastQC Report
Mon 27 Feb 2023
SRR3127906.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127906.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3529565
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT573641.625242770709705No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG36440.10324218423516779No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT179300.086.988111
CGTAGGG10000.075.6681442
TAGGGCA16000.074.3169254
ATAGGGC20750.072.253653
GAATAGG21600.070.117731
ATAGGGA32400.069.627923
AGGGATG44450.068.832955
GAGGGAT44200.067.4146354
AGTAGGG56100.066.351312
TAGAGGG66600.065.6995852
AGGGCAT29450.065.431335
TAGGGAT24050.065.0753254
TATAGGG26900.064.819972
TGTAGGG30150.064.223962
AAGAGGG118800.064.12892
AATAGGG56250.064.085542
AGAGGGA82350.064.034863
TAAGGGA31800.063.995283
GTACGGG9850.063.937522
ACGGGAT10150.063.899914
Produced by FastQC (version 0.11.5)