FastQCFastQC Report
Mon 27 Feb 2023
SRR3127905.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127905.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2745235
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343901.252716069844658No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38530.14035228313787346No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31120.11336005842851339No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG29030.10574686684382212No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG27480.1001007199747927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102500.085.6558761
ACGGGTA2600.081.344044
AGGGATG54350.077.4809655
TAGGGCA13250.075.907464
CGTAGGG8350.075.456172
ATAGCGG6250.074.528881
AGAGGGC38400.071.966893
AGGGCAT20900.071.0603565
GAGGGAT39750.070.468614
ATAGGGA26750.070.278553
GGTAAGG15600.069.973361
AGTAGGG45200.069.800832
ATAGGGC18100.069.5893553
GAATAGG16200.069.4148251
GTAGGGA22150.068.959843
AAGGGAT51950.068.666174
AGGGAAT32900.068.4267965
AAGAGGG95850.068.3828352
TAAGGGA28700.068.287433
TAGGGAT21300.066.857424
Produced by FastQC (version 0.11.5)