FastQCFastQC Report
Mon 27 Feb 2023
SRR3127904.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127904.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566510
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309281.2050605686321114No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34390.13399519191431164No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG30630.11934494702923425No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG29720.11579927605970754No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC27400.10675976325827681TruSeq Adapter, Index 16 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27260.10621427541681115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95200.086.120951
ATAGCGG5150.076.7220841
TAGGGCA12350.076.488574
AGGGATG49250.075.385595
CGTAGGG7550.075.322162
AGAGGGC38250.073.600333
AGTAGGG41200.072.6652152
ATAGGGA24000.072.4560853
ACGGGTA2200.070.495064
GAGGGAT35450.070.263064
ATAGGGC18150.070.1741263
GTAGGGA19050.070.066063
TAGGGAT20500.069.692684
AAGAGGG91900.069.398272
AGGGAAT29900.069.316215
TAAGGGA26000.068.690173
AGGGCAT19900.068.2514955
GAGGGTA12650.067.6158144
TAAGAGG19350.067.579061
ATAGAGG18200.067.197071
Produced by FastQC (version 0.11.5)