FastQCFastQC Report
Mon 27 Feb 2023
SRR3127903.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127903.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3195548
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441511.3816409579827935No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46230.1446700221683417No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35210.11018454424718388No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT135050.085.94741
AGGGATG64750.077.6675955
ACGGGTA3000.076.763914
ATAGGGC19900.073.921973
ATAGCGG6300.072.441821
TAGGGCA14800.072.40284
CGTAGGG11800.071.2940442
TATAGGG25700.070.406042
AGTAGGG53500.070.102162
TAGAGGG57000.069.5080262
GAGGGAT50100.069.231044
AAGAGGG114300.069.122
TAAGGGA34050.068.875653
ATGCGGG17100.068.4361342
AGAGGGC43950.068.225083
GTAGGGT11750.067.997563
GGATGGC54250.067.9222647
ATAGGGA31700.067.902923
ATAACGG3750.067.751771
AAGGGAT60600.067.395274
Produced by FastQC (version 0.11.5)