FastQCFastQC Report
Mon 27 Feb 2023
SRR3127902.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127902.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114288
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT801823.792387792013198No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT71910.3401144971735166No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT68260.3228510023232407No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT64950.30719561384257965No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG40480.1914592524764838No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33110.15660118205277615No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC31630.14960118962033556No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC30570.14458768152683077No Hit
CGTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC25610.1211282474289217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT231650.090.114981
TAGGGTA8250.075.767074
ATAGGGC14000.075.197393
AGGGTAC10100.073.52225
AGGGATG29900.072.4623645
AGTAGGG34000.071.901962
GTAGGGT10250.071.07073
TAGGGTC6700.070.146814
AGGGAAT25200.070.124555
AGAGGGC31500.069.080153
TAGGGCA9700.068.3172154
ACGGGTA3100.068.223444
AGATAGG12250.067.994681
ATAGCGG4850.067.919431
ATAGGGA18650.067.284523
ACGGGAT5400.067.016194
TGTAGGG14800.066.0720752
AAGGGTA14700.065.8616644
TAGAGGG39250.065.7580642
GTTTTTT321350.065.599972