FastQCFastQC Report
Mon 27 Feb 2023
SRR3127901.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127901.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1996260
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT739903.706431026018655No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT77080.3861220482301904No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT73010.36573392243495334No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT69400.34765010569765464No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG42350.21214671435584542No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC35490.17778245318746055No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC33960.17011812088605693No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29430.14742568603288148No Hit
CGTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC27770.1391101359542344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT209850.090.028061
ATAGGGC13600.076.020493
AGGGATG28650.074.143265
TAGGGTA7700.073.2363364
TAGGGCA9100.072.813814
GTAGGGA13200.071.203773
AGTAGGG30100.070.257542
ATAGCGG3800.069.326231
AGTAAGG11450.068.612641
GTAGGGT7150.068.355623
ATAGGGA16100.068.302553
GAATAGG12600.067.5773541
AGAGGGC30600.067.573783
ACGGGTA2300.067.4251864
CGTAGGG5950.067.134992
AGGGCAT13450.067.086755
ATAGGGT8850.066.907413
GATAGGG27850.066.821612
TATAGGG14950.066.641022
AGGGAAT20200.066.5384145