FastQCFastQC Report
Mon 27 Feb 2023
SRR3127900.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127900.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469061
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1026874.158949495374962No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT76920.3115354379660932No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT76030.30793082876445743No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT68710.2782839306116779No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG44780.181364494437359No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37340.15123158156076338No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC36510.14786997972103566No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC31530.12770036868266924No Hit
CGTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC29010.11749405948253203No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25460.10311612390297364No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT286350.089.4600451
ATAGGGC15650.075.383053
GACCGAT2250.073.110928
AGTAGGG39900.071.739562
AGGGTAC12100.070.694045
AGGGATG36800.070.627535
GAATAGG14650.069.352931
TAGGGCA11950.068.831064
GTAGGGT9800.068.5842363
AGATAGG14800.068.33221
AGGGAAT25700.068.030955
TAGGGTA8950.067.220434
AGAGGGC38250.067.2156143
AGGGACT28400.067.189975
ATAGGGA21150.066.8914263
GTTTTTT387600.066.28272
GAGGGAT26100.066.270714
GATAGGT6250.066.1758358
AGTAAGG14950.066.073421
CGTTTCT12400.066.004911