FastQCFastQC Report
Mon 27 Feb 2023
SRR3127899.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127899.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107555
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104810.9463186929768724No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC51140.4617377918026644No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG47130.425531914893617No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC44320.4001607143663295No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG42070.37984569615052977No Hit
AGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT17270.15592905092749343No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT15560.14048963708348572No Hit
TAGACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT13670.12342502178221398No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT12570.11349323509893415No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT12390.11186803364167017No Hit
GGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT12090.10915936454623022No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTTG11820.10672156236033424No Hit
ACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG11750.10608953957139827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT1300.090.379415
ACGGGTA1600.088.119924
CGTTTTT31650.086.061571
AGTACGG1850.083.916341
AGGGATG27750.083.324925
GGCCGAT1250.082.715237
CGCTACG8150.082.46153
GCTACGA8150.082.46154
ATAGCGG1950.082.025461
CGTAGGG3500.081.931762
AGGGTAC4600.081.734425
TATCGGG1100.081.1991352
CGAAGGG9450.080.0911562
GCGTAGG1850.078.8305051
AGGGAAT23550.078.827735
AGGGTAT8900.078.6808555
GCGGGAT5500.078.613654
CTACGAC8550.078.603655
TAGAGGG18000.078.3500442
GATCGGT301.4566049E-678.328828