FastQCFastQC Report
Mon 27 Feb 2023
SRR3127898.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127898.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023604
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94510.9233062785999273No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG51130.4995095759688317No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC47000.4591619415320769No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46770.456914978839473No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG44660.4363015384855862No Hit
AGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT18080.17663080644467977No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT17240.16842450791517033No Hit
TAGACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT14130.1380416645499627No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT13990.13667394812837777No Hit
ACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG13310.13003075408067963No Hit
GGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT13020.1271976272073966No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT12530.12241061973184943No Hit
ACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT11830.11557203762392487No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTTG11310.11049194805803807No Hit
TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG10940.10687726894384939No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT1000.093.985395
ACGGGTA1650.091.1373444
CGTTTTT30850.089.015151
ATAGCGG1850.086.419831
TAGCGGG3100.084.890032
CGCTACG8050.084.061473
CGAGGGA5450.083.638383
AGGGATG25150.082.587565
GCGATTG401.036824E-982.2452558
GCTACGA8300.082.095684
CGTAGGG2650.081.572232
CGGGTAA1100.081.1692055
TCCGCTA8900.079.779851
CTACGAC8550.079.695225
GGAATGC11000.079.468157
GGGAATG25750.079.38966
AGGGAAT22200.079.3795555
GGGCGAT3850.079.34226
GAGGGAT18850.079.276814
AGGGTAT7900.079.114295
Produced by FastQC (version 0.11.5)