FastQCFastQC Report
Mon 27 Feb 2023
SRR3127897.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127897.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234047
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137031.1104115159309167No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC59510.4822344691895851No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC49050.3974727056586986No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG47480.38475033770999No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG46540.37713312377891606No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT18360.1487787742282101No Hit
AGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT17440.1413236286786484No Hit
TAGACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT14660.11879612364845099No Hit
ACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG13460.1090720207577183No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT12650.10250825130647374No Hit
ACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT12470.10104963587286384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT41050.086.195111
TATCGGG1100.085.455472
CGCTACG11500.084.6009143
GCTACGA11550.084.234674
AGGGATG33950.084.1683355
ATAGCGG2000.082.340971
GGATGGC25300.080.622117
TCCGCTA12400.080.443711
CTACGAC12250.079.4180455
TACGACC12250.079.034386
AGGGTAT9050.078.9368745
GTAGGGC6050.078.463663
CGACCAA12150.078.1375968
AAGGGCG5600.078.054414
AGGGAAT26500.078.0354165
CGAGGGA7150.077.567273
GAGGGAT26600.077.3918154
GATGGCA24300.077.170558
CGTAGGG3250.076.646972
GCGAGGG8900.076.573862