FastQCFastQC Report
Mon 27 Feb 2023
SRR3127896.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127896.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780270
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308951.1112230107147867No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG27850.10017012736173105TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96950.084.696611
ATAGGGC18150.075.352133
AGGGTAC13150.072.194615
AGTAGGG46450.070.9512252
GTACGGG7150.070.3566742
TATAGGG24150.069.1096652
AAGGGTA20800.068.689354
GAATAGG18300.067.3703161
ATAGGGA25250.067.1931463
TAGACGG2750.066.734491
TAGAGGG52300.066.520822
TAGGGCA14900.065.923334
ATAACGG2650.065.701341
ATAGAGG19700.065.687821
TAGGGTA11450.065.6740344
TAGCGGG15400.065.636482
ATAAGGG39050.065.25382
ATAGCGG5050.065.2266541
AAGAGGG99550.065.125432
ATGAGGG42250.064.873812
Produced by FastQC (version 0.11.5)