FastQCFastQC Report
Mon 27 Feb 2023
SRR3127895.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127895.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2580000
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277311.0748449612403101No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG30200.11705426356589148TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG27500.1065891472868217TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC26310.1019767441860465TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89200.085.3741761
CGTAGGG7550.075.318912
AGGGTAC12350.071.162095
ATAGCGG5200.070.55631
AGTAGGG38050.069.5375442
ATAGGGC16300.069.197313
AAGGGTA19200.068.291814
ATAGGGA24700.067.926133
GAATAGG16650.067.519351
AGATAGG15900.067.154211
AGTAAGG16550.066.790451
ACGGGTA2400.066.578394
TAGGGTA9750.066.518144
TAGAGGG44950.066.077412
AGAGGGC38950.065.758923
AATAGGG40050.065.712972
AGGGATG31200.065.374625
ATAAGGG36150.065.26212
TATAGGG22000.065.154252
GTACGGG5850.065.072092
Produced by FastQC (version 0.11.5)