FastQCFastQC Report
Mon 27 Feb 2023
SRR3127894.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127894.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3223028
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT392231.2169611930147675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT119400.085.9320761
TAGGGTA12900.073.2317054
ATAGGGC18450.073.110253
AGGGTAC14800.072.407935
TATAGGG27500.070.5846252
ATAGGGA35500.070.168193
AGTAAGG22300.069.82761
AAGGGTA23600.069.3042764
TAGACGG3600.069.259181
GTACGGG7550.069.0985262
AGTAGGG52350.067.154892
ATAGAGG21600.066.863421
AGGGAAT36300.066.4238055
AAGAGGG110200.066.0212
AGTACGG4000.065.861561
ATAGCGG7800.065.740931
AGGGATG41700.065.486695
TAGAGGG59350.065.4111942
AGGGTAT20050.065.403785
ATAACGG3750.065.23431
Produced by FastQC (version 0.11.5)