FastQCFastQC Report
Mon 27 Feb 2023
SRR3127893.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127893.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2646568
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377321.4256954667327648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115250.085.875831
ATAGGGC17450.073.7938463
ACGGGTA4250.072.982724
AGTAGGG46700.072.094012
AGGGATG43550.071.115175
AGGGTAC14350.071.06785
ATAGCGG5800.069.7819441
TAGGGTA12550.069.277634
GTAGGGT12400.068.978663
AAGGGTA21900.068.670614
TAAGGGA21100.068.155993
ATAACGG3050.067.8930741
TAGGGCA15800.067.520214
ACGGGAT7750.067.311044
GTAAGGG38900.067.209132
TAGAGGG44800.066.544822
ATAGGGA26700.066.53433
AAGAGGG88850.066.524382
ATGCGGG11150.066.210652
TAGGGTC7950.066.208834
Produced by FastQC (version 0.11.5)