FastQCFastQC Report
Mon 27 Feb 2023
SRR3127892.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127892.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439991
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339171.3900461108258186No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG26640.10918073058466199No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG26620.10909876306920804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT101650.086.7948761
ACGGGTA2600.079.5326164
AAGGGTA19150.070.924294
AGGGATG38750.070.82985
AGATAGG12750.070.820611
ATAGGGA21600.070.494823
ATAGCGG4750.070.296451
AGGGTAC12250.069.824875
AGTAGGG41750.069.4535752
ATAGGGC16150.069.258073
TAGCGGG12050.069.032272
CGTAGGG6050.068.358612
ATAAGGG35100.067.616122
TAGGGTA9400.067.495034
AGGGAAT26900.067.438815
AGTAAGG15050.067.184741
GTAAGGG36550.067.119562
GTAGGGA21500.066.6695253
AACGAGG10050.066.449381
AGGGTAT14800.066.368115
Produced by FastQC (version 0.11.5)